Riboflavin Biosynthesis byCandida robusta

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Biosynthesis of Riboflavin.

The biosynthesis of riboflavin requires 1 equivalent of GTP and 2 equivalents of ribulose phosphate. The first committed reactions of the convergent pathway are catalyzed by GTP hydrolase II and 3,4-dihydroxy-2-butanone 4-phosphate synthase. The initial reaction steps afford 5-amino-6-ribitylaminopyrimidine 5'-phosphate, which needs to be dephosphorylated by a hitherto elusive hydrolase. The de...

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Some nutritional requirements for biosynthesis of riboflavin by Eremothecium ashbyii.

Eremothecium ashbyii Guilliermond is a remarkable organism because of its capability of synthesizing relatively large amounts of riboflavin under appropriate conditions. This vitamin is synthesized to a limited extent by a rather wide variety of microorganisms (Pridham, 1952), but those known particularly for their riboflavin producing abilities along with E. a&hbyii are Ashbya gosypi'i (Wicker...

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Biosynthesis of Riboflavin Preparation of Phosphorylated Pyrimidine Intermediates

2-Am ino-5-nitro-6-(r-D -ribityl)am ino-4(3H )-pyrim idinone (6a) was converted to the dimethoxytrityl derivative, which was subsequently acetylated. The trityl residue was then rem oved, and the compound was phosphorylated by the cyanoethyl method. Rem oval of the protecting groups yielded 2-am ino-5-nitro-6-(l'-D-ribityl)am inopyrim idinone 5'-phosphate (11a). Catalytic hy­ drogenation yielde...

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Biosynthesis of anthraquinones in cell cultures of Cinchona 'Robusta' proceeds via the methylerythritol 4-phosphate pathway.

Robustaquinone B was found as a major anthraquinone in cell cultures of Cinchona 'Robusta' after treatment with a fungal elicitor. Anthraquinones in Cinchona are considered to be of the Rubia type, i.e. rings A and B are derived from chorismate and alpha-ketoglutarate, whereas ring C is formed from isopentenyl diphosphate (IPP). To determine the origin of IPP, either formed via the mevalonic ac...

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Effects of environmental conditions on production of xylitol byCandida boidinii.

Candida boidinii NRRL Y-17213 produced more xylitol thanC. magnolia (NRRL Y-4226 and NRRL Y-7621),Debaryomyces hansenii (C-98 M-21, C-56 M-9 and NRRL Y-7425), orPichia (Hansenula) anomala (NRRL Y-366). WithC. boidinii, highest xylitol productivity was at pH 7 but highest yield was at pH 8, using 5 g urea and 5 g Casamino acids/I. Decreasing the aeration rate decreased xylose consumption and cel...

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ژورنال

عنوان ژورنال: Agricultural and Biological Chemistry

سال: 1964

ISSN: 0002-1369

DOI: 10.1080/00021369.1964.10858272